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Sir
Contrary to supposition by Masahiro Iizuka and colleagues (July 8, p 160),1 our data from molecular virological studies examining the role of measles virus infection in children with autism and enterocolitis have been peer-reviewed, presented, and published at four international scientific meetings.2-4 We would have been happy to share this information with Iizuka and colleagues if asked. The antibody used in our studies is not that used by Iizuka and colleagues. For the purpose of clarification, our studies involved the use of in-situ hybridisation, in-cell reverse transcriptase, real-time quantitative Taq Man PCR using complementary RNA standards for quantitation and sequencing of complementary DNA measles virus amplicon. In addition, three genes N, F, and H of measles virus were examined. We have also provided data in relation to sub-cellular localisation of measles virus, concordant with the molecular biological findings.
References
- Iizuka M, Itou H, Chiba M, et al. The MMR question. Lancet 2000; 356: 160.
- Wakefield AJ, Anthony A, Sim R, et al. Persistent measles virus infection immunodeficiency in children with autism ileo-colonic lymphoid nodular hyperplasia and non-specific colitis [suppl]. Gut 1998; 42: A86
- Uhlmann V, Martin C, Sheils O, et al. Measles virus in reactive lymphonodular hyperplasia and ileo-colitis in children [suppl]. J Pathol 2000; 190: 1A-69A
- Uhlmann V, Sheils O, Leittich K, et al. Identification of measles virus genomes in ileo-colonic lymphoid hyperplasia in children. Lab Invest (in press).
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